1.3.7.4 - 437673 1.3.7.4 50000fold 437768 1.3.7.4 90% homogeneity 437767 1.3.7.4 HPLC Phenomenex Ultracarb reverse phase column chromatography 685125 1.3.7.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration. The PPhiB synthase monomer is contained in the major peak of anion-exchange chromatography, and the PPhiB synthase homodimer in the minor peak, for purification, the monomeric major peak fraction is used 763272 1.3.7.4 recombinant ZmHy2 657069 1.3.7.4 Superdex 200 gel filtration 687760