1.3.1.84	native enzyme by anion exchange and hydrophobic interaction chromatography, ultrafiltration, hydroxyapatatite chromatography, and again ultrafiltration. The enzyme is separated from acryloyl-CoA hydratase	745800	
1.3.1.84	Ni-NTA column chromatography and Superose 12 gel filtration	740623	
1.3.1.84	recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2(DE3) by immobilized metal affinity chromatography and gel filtration	745689	
1.3.1.84	recombinant His10-tagged enzyme 11fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration	745213	
1.3.1.84	recombinant His10-tagged enzyme 4fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration	745213	
1.3.1.84	recombinant His10-tagged enzyme 6fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration	745213