1.2.1.49 - 1.2.1.49 using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200 1.2.1.49 using ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50 column, Blue-dextran affinity column and CM-Sephadex C-50 column 1.2.1.49 using ammonium sulfate fractionation and chromatography on DEAE-Sephadex column, Blue-dextran affinity column and CM-Sephadex column 1.2.1.49 using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose 1.2.1.49 using treatment with acetone, ammonium sulfate fractionation, and gel filtration on Sephadex G-25