1.2.1.26 - 1.2.1.26 by gel filtration 1.2.1.26 KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration 1.2.1.26 KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration 1.2.1.26 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-T1 by nickel affinity chromatography, gel filtration, and ultrafiltration 1.2.1.26 recombinant N-terminally His6-tagged wild-type and mutant KGSADH enzymes from Escherichia coli strain DH10beta by nickel affinity chromatography and ultrafiltration 1.2.1.26 to homogeneity