1.2.1.19 - 288041, 288078, 288079 1.2.1.19 ammonium sulfate, DEAE Sephacel, hydroxyapatite, 5'-AMP Sepharose 4B, Mono Q, TSK-GEL 656627 1.2.1.19 cobalt- or nickel-charged IDA-Sepharose column chromatography and Resource Q column chromatography 712753 1.2.1.19 from putrescine-grown cells, using ammonium sulfate fractionation and column chromatography on Sephacryl S-300, DEAE-Sephacel and Blue-Sepharose CL6B 288063 1.2.1.19 from putrescine-grown cells, using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, Sephadex G-200, Hydroxylapatite and Sephadex G-200 in 150 mM NaCl 288065 1.2.1.19 native enzyme 27.22fold from germinated seeds by ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography 742430 1.2.1.19 recombinant His-tagged enzyme from in Escherichia coli by affinity chromatography 743820 1.2.1.19 recombinant His-tagged isozyme AMADH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 763552 1.2.1.19 recombinant His-tagged isozyme AMADH2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 763552 1.2.1.19 to homogeneity by ammonium sulfate precipitation, anion-exchange chromatography and hydrophobic interaction chromatography 661760 1.2.1.19 using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, AMP-Sepharose, Sephadex G-200 and NAD+-Sepharose 288064 1.2.1.19 using CM-trisacryl, DEAE-Sephacel, 5'-AMP-Sepharose affinity chromatography, and fast protein liquid chromatography on a mono-Q column 288080 1.2.1.19 using column chromatography on DEAE-cellulose 288077 1.2.1.19 using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue 288069 1.2.1.19 using column chromatography on DEAE-cellulose, hydroxylapatite, Sephacryl S-300 and Phenyl-Superose and isoelectrofocusing 288081 1.2.1.19 using column chromatography on hydroxylapatite, precipitation with ammonium sulfate, column chromatography on Sephacryl S-300, Phenyl-superose, 5'-AMP-Sepharose and hydroxylapatite 288082 1.2.1.19 using protamine, ammonium sulfate, acetone and column chromatography on DEAE-cellulose 288066