1.14.11.8	-	
1.14.11.8	partial	
1.14.11.8	recombinant active and stable His- and MBP-tagged TMLH from Escherichia coli strain BL21-AI by nickel or amylose affinity chromatography. It is possible that the His6-tag at the N-terminus of the fusion protein is masked and cannot be accessed by the Ni-beads. Thus, MBP affinity purification is selected as a first purification step, followed by gel filtration, His6-MBP-tag cleavage by recombinant TEV protease	
1.14.11.8	recombinant MBP-taged enzyme by affinity chromatography, tag cleavage by rTEV protease, and gel filtration