1.13.12.5	-	
1.13.12.5	by affinity chromatography	
1.13.12.5	Hi-Trap column	
1.13.12.5	natively folded GLuc is obtained by bacterial expression and efficient refolding using a solubility enhancement petide tag	
1.13.12.5	recombinant His-tagged engineered enzyme C-SRLuc8 in Escherichia coli by nickel affinity chromatography	
1.13.12.5	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
1.13.12.5	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography to over 95% purity	
1.13.12.5	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
1.13.12.5	recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	
1.13.12.5	recombinant secreted enzyme	
1.13.12.5	recombinant soluble His6-tagged enzyme FcUni-RLuc from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography	
1.13.12.5	souble and insoluble fraction by nickel-chelate column	
1.13.12.5	using Ni-chelate column