1.11.1.18 - 671929, 671956, 672433, 675172, 675244, 675246, 675819, 676361, 689414, 699389, 726562 1.11.1.18 ammonium sulfate precipitation, Ni-NTA column chromatography, and DE52 gel filtration 742111 1.11.1.18 BPO 1 675173 1.11.1.18 BPO 2 675173 1.11.1.18 isolation of three forms of CoVBPO: nVBPO which is isolated directly from the algal species, sVBPO which is produced in a soluble form by Escherichia coli and rVBPO which is expressed by Escherichia coli as inclusion bodies and subsequently refolded 676980 1.11.1.18 Na3VO4 is added to the crude extract (1.12 mg protein/ml) followed by heat treatment at 70°C for 2 h, 30-55% (w/v) ammonium sulfate precipitation and DEAE-52column chromatography 711546 1.11.1.18 native enzyme VBrPO(AnII) from algal powder by liquid-liquid-partitioning, dialysis, and ultracentrifugation 763808 1.11.1.18 Ni2+-charged HiTrap chelating column chromatography 711201 1.11.1.18 nonhaem-type bromoperoxidase BPO1a 675171 1.11.1.18 nonhaem-type bromoperoxidase BPO1b 675171 1.11.1.18 nonhaem-type bromoperoxidase BPO3 675171 1.11.1.18 phenyl Sepharose column chromatography and Phenyl Sepharose gel filtration 700668 1.11.1.18 recombiant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation and nickel affinity chromatography followed by desalting gel filtration 764075 1.11.1.18 recombinant enzyme from Pichia pastoris strain X-33 by ultrafiltration, anion exchange chromatography, dialysis, and gel filtration 751538