1.1.99.31	-	
1.1.99.31	His6-tagged enzyme, Ni-resin column chromatography and Sephadex G-25 gel filtration	
1.1.99.31	mutant enzymes H274G, H274A and H274N	
1.1.99.31	mutant enzymes R277K, R277G, R277H and R277L	
1.1.99.31	recombinant enzyme	
1.1.99.31	Toyopearl DEAE-650 M column chromatography	
1.1.99.31	Toyopearl DEAE-650M column chromatography	
1.1.99.31	wild-type and mutant enzymes