1.1.1.406 recombinant His(6)-tagged GatDH from Escherichia coli strain BL21GOLD(DE3) by use of Co2+-IDA functionalized Fe2O3 nanoparticles, purification and immobilization method, overview 1.1.1.406 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 1.1.1.406 recombinant His6-tagged GatDH from Escherichia coli strain BL21(DE3), by nickel affinity chromatography the tag is cleaved off, followed by dialysis and gel filtration 1.1.1.406 to homogeneity, chromatography steps