1.1.1.324 native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration 1.1.1.324 native enzyme to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, anion exchange, and AF-Red dye affinity chromatography followed by gel filtration