3.4.21.48 proteolytic modification the proteinase B precursor is glycosylated and proteolytically processed at least three times before mature enzyme is formed. The precursor is autocatalytically cleaved to yield the NH2 terminus and the COOH terminus of the mature enzyme. The pro region plays an essential role in the proteolytic processing of the enzyme 95515 3.4.21.48 side-chain modification Asn-linked glycosylation 95514 3.4.21.48 side-chain modification contains 8-9% neutral sugars and 1.5% amino sugars 95499