2.3.1.164 additional information ptoIAT overexpressed in Escherichia coli is not self-processed even when treated with Aspergillus nidulans crude extract 658618 2.3.1.164 proteolytic modification recombinant expression in Hansenula polymorpha give a 40 kDa pre-protein that is processed into an 11 and 29 kDa subunit 673752 2.3.1.164 proteolytic modification the intronless gene is expressed in Escherichia coli and self-processed efficiently into its alpha and beta subunits 658618 2.3.1.164 proteolytic modification the proenzyme is processed autocatalytically to generate two polypeptides forming alpha-subunit and beta-subunit 720282