2.7.8.43 phosphoprotein phosphorylation of the putative nucleophile, Thr280 734476 2.7.8.43 phosphoprotein the cEptC active site contains a putative nucleophilic threonine, Thr266, covalently bound to a phosphoryl group to form phosphothreonine. Phospho-Thr266 mimics an enzyme reaction intermediate representing the first step in a two-step transferase reaction -, 739786