3.4.24.25	proteolytic modification	enzyme is synthesized as a preproenzyme. The propeptide inhibits the mature enzyme	
3.4.24.25	proteolytic modification	the MvP1 propeptide undergoes both N-terminal and C-terminal processing and different C-terminal processing results in the formation of several active isoforms of the mature peptidase	
3.4.24.25	proteolytic modification	recombinant pro-vibriolysin is cleaved to the 35 kDA mature vibriolysin	
3.4.24.25	proteolytic modification	secretion of HA/protease into the extracellular medium is a two stage process. First, the preproprotein or primary translation product is translocated through the cytoplasmic membrane with removal of its signal peptide by a Sec-dependent mechanism. Second, the proprotein folds in the periplasmic space and is secreted across the outer membrane via the type II secretion system, a multiprotein complex encoded by 12 eps (extracellular protein secretion) genes. Removal of the HA/protease propeptide and C-terminal processing is presumed to occur in the extracellular medium through an autocatalytic mechanism