Bacillus licheniformis - 135338 Bombyx mori silkworm 135337 Bos taurus - 135316, 135318, 135327, 135328, 135344, 135345, 135350, 135351, 135352, 135353, 135354, 135355, 135357, 135360, 135361, 135365, 135366, 135367, 649785, 650012, 668704, 670144, 670330, 693333, 703570, 711849, 716198, 716199, 716349 Bos taurus isoform PDE5 681583 Bos taurus isoform PDE6 680648, 680735, 682655 Bos taurus methods of enzyme determination 135329 Bos taurus PDE6 668987 Bos taurus PDE6 PDE6 668987 Canis lupus familiaris - 651222, 653097, 668485 Cavia porcellus - 135333, 667545, 692697 Danio rerio - 693977 Dictyostelium strains AX3 and DH1 653180 Dictyostelium discoideum - 649701 Dictyostelium discoideum isoforms PDE5, PDE6 678053 Dictyostelium discoideum strains NC4, XP55 and NP377 653179 Drosophila melanogaster - 667501, 690833 Drosophila sp. (in: flies) generation of transgenic animals with targeted expression of DmPDE6 to tubule type I (principal) cells. This reveals localization of DmPDE6 primarily at the apical membranes. Overexpression of DmPDE6 results in elevated cG-PDE activity and decreased tubule cGMP content. Such targeted overexpression of DmPDE6 creates a novel phenotype that manifests itself in inhibition of the active transport and efflux of cGMP by tubules 667521 Gallus gallus - 669288, 669954, 693132 Homo sapiens - 650811, 651322, 652763, 653097, 653343, 667119, 667433, 668990, 669021, 669209, 670025, 670287, 670332, 670983, 671011, 691533, 691945, 692724, 693333, 694879, 710776, 710867, 711409, 711553, 711554, 711557, 711619, 711624, 711652, 711841, 711934, 711948, 712091, 712187, 712394, 712630, 712671, 712841, 713068, 713513, 713514, 750045, 751213 Homo sapiens isoenzymes PDE5A1, PDE5A2 and PDE5A3 649549 Homo sapiens isoform PDE5 667433, 670983, 679253, 679518, 679676 Homo sapiens isoform PDE5A 678339, 678726 Homo sapiens isoform PDE5A1 678243 Homo sapiens isoform PDE6 680637 Homo sapiens isoforms PDE5, three splice variants: PDE5A1, PDE5A2, PDE5A3. Enzyme promoters are sensitive to cGMP or cAMP stimulation 679401 Homo sapiens PDE5 668987 Homo sapiens PDE5A 692191, 716347 Homo sapiens PDE9 668987 Homo sapiens PDE9A 716347 Homo sapiens recombinant and native 667547 Homo sapiens recombinant PDE5A1 670642 Homo sapiens splice variants PDE5A1 and PDE5A2 650813 Homo sapiens splice variants PDE9A5 and PDE9A1 651412 Lithobates catesbeianus - 135349, 135352 Lithobates catesbeianus bullfrog 135319, 135359 Lithobates grylio - 135359 Lithobates pipiens - 652318 Mus musculus - 135278, 135363, 650342, 652975, 670332, 670387, 691967, 692449, 692703, 693110, 711925, 712364, 712975, 714691, 715716, 716349, 729558, 750479 Mus musculus isoform PDE6 681542 Mus musculus C57/BL6J - 715716 Mycobacterium tuberculosis variant bovis - 135322 Mycolicibacterium smegmatis - 135322 no activity in Homo sapiens functional PDE6 not detected in Y79 retinoblastoma cells. Attempts to enhance iexpression with cell culture or with introduction of virus expressing PDE6 are not successful. The results indicate that expression of a fully active stable PDE6 enzyme requires other post-transcriptional events that do not occur or are inhibited in Y79 cells 670344 Oryctolagus cuniculus - 653097, 670287, 713572 Oryctolagus cuniculus isoform PDE5 678974 Plasmodium falciparum - 667518, 713049 Rana sp. frog 653124 Rattus norvegicus - 135314, 135340, 649520, 650180, 652975, 653083, 667126, 668269, 668864, 669232, 670145, 691558, 694285, 711555, 711626, 711687, 711744, 711772, 711773, 711792, 711829, 711920, 711923, 712621, 712700, 712815, 713199, 713217, 715153, 729558, 750479 Rattus norvegicus isoform PDE5 678445, 679253, 679518, 679666 Rattus norvegicus isoforms PDE5, PDE9 681045 Rattus norvegicus pregnant rat 677491 Rattus norvegicus spontaneous hypertensive rats 650737 Rattus norvegicus Sprague-Dawley - 715153 Rhinella marina - 135359 Strongylocentrotus purpuratus - 670209 Strongylocentrotus purpuratus sea urchin 135368 Sus scrofa - 678907, 692087 Sus scrofa fragment 692087 vertebrata overview 135315