3.2.1.105	medicine	plays central role in indole alkaloid biosynthesis	667805	
3.2.1.105	pharmacology	Catharanthus roseus produces many pharmaceutically important terpenoid indole alkaloids (TIAs) such as vinblastine, vincristine, ajmalicine, and serpentine. Transcriptional regulator ORCA3 and strictosidine glucosidase (SGD) are both engineered in hairy roots under the control of a glucocorticoid-inducible promoter. Co-overexpression of ORCA3 and SGD result in a significant increase in serpentine by 44%, ajmalicine by 32%, catharanthine by 38%, tabersonine by 40%, lochnericine by 60% and hörhammericine by 56% . The total alkaloid pool is increased significantly by 47%. Combining overexpression of a positive regulator and a pathway gene which is not controlled by this regulator provides a way to enhance alkaloid production	752145