1.1.1.202	industry	development of an economical and eco-friendly biological process for the production of propane-1,3-diol from renewable resources by construction of a novel operon including YqhD	-, 695764	
1.1.1.202	synthesis	constitutive overexpression of 1,3-PD oxidoreductase in Klebsiella pneumoniae leads to a nearly 3fold decrease in peak level of the intermediary metabolite 3-hydroxypropionaldehyde and an increase of 16.5% in yield of 1,3-propanediol with respect to the wild-type strain	695716	
1.1.1.202	synthesis	Escherichia coli is engineered to produce 1,3-propanediol from glycerol, an inexpensive carbon source. This is done by introducing a synthetic 1,3-propanediol production pathway in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli. When 10 mM succinate is added to the medium, the titer of 1,3-propanediol and the glycerol consumption increase to 9.9 and 23.84 g/l, respectively. In addition, the ratio of NADH to NAD+ increases by 43%. Succinate addition is a promising route for the biotechnological production of NADH-dependent microbial metabolites	747304	
1.1.1.202	synthesis	expression of the yqhD gene, encoding 3-propanediol oxidoreductase isoenzyme from Escherichia coli and the dhaT gene, encoding 3-propanediol oxidoreductase from Klebsiella pneumoniae individually and concomitantly in Klebsiella pneumoniae using the double tac promoter expression plasmid pEtac-dhaT-tac-yqhD. The three resultant recombinant strains show that the peak values for 3-hydroxypropionaldehyde production in broth of the three recombinant strains are less than 25% of that of the parent strain. Expression of dhaT reduces formation of by-products ethanol and lactic acid and increases molar yield of 1,3-propanediol slightly, while expression of yqhD does not enhance molar yield of 1,3-propanediol, but increases ethanol concentration in broth as NADPH participation in transforming 3-hydroxypropionaldehyde to 1,3-propanediol allows more cellular NADH to be used to produce ethanol. Co-expression of both genes therefore decreases by-products and increases the molar yield of 1,3-propanediol by 11.8%, by catalyzing 3-hydroxypropionaldehyde conversion to 1,3-propanediol using the two cofactors NADH and NADPH	710972	
1.1.1.202	synthesis	genetic engineering of Klebsiella pneumoniae for production of 1.3-propanediol from glycerol. Constitutive expression of the dhaT gene alone gives the highest yield, fed-batch fermentation results in efficient production of 1,3-propanediol from either pure or crude glycerol, without by-product formation	724566	
1.1.1.202	synthesis	Klebsiella pneumoniae is used for production of 1,3-propanediol by the enzyme which is utilized in plastic industry	-, 657406	
1.1.1.202	synthesis	NADH-dependent 1,3-propanediol oxidoreductase is a key enzyme for the production of 1,3-propanediol in soluble cell extract. Klebsiella pneumoniae J2B shows a high potential for the production of 1,3-propanediol from glycerol. Optimization of the culture conditions and the elimination of lactate synthesis improves 1,3-propanediol production significantly	-, 747304	
1.1.1.202	synthesis	the enzyme can be used for 1,3-propandiol synthesis for use in resaerch and industrial applications, especially in biodiesel industry, but also as a monomer for polycondensation to manufacture plastics with special properties, i.e., polyesters, polyethers, polyurethanes, and polytrimethylene terephthalate as a monomer for cyclic compounds, and as a polyglycol-type lubricant	-, 740214	
1.1.1.202	synthesis	the enzyme is useful in 1,3-propanediol production from glycerol, a byproduct in biodiesel production, by an enzymatic bioconversion in a membrane reactor in which the NAD+ coenzyme can be regenerated, mathematical description and modelling of the system, overview	684524