3.5.5.5 synthesis nitrilases are important industrial enzymes that convert nitriles directly into the corresponding carboxylic acids -, 719629 3.5.5.5 biotechnology preparation of optically pure carboxylic acids -, 694107 3.5.5.5 synthesis recombinant cells expressing the enzyme are promising catalysts for the synthesis of stable chiral quaternary carbon centers from ketones -, 718648 3.5.5.5 synthesis the enzyme is promising for the enantioretentive transformation of (S)-mandelonitrile 757638 3.5.5.5 synthesis the whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 is employed to develop a bioprocess for the synthesis of 4-hydroxyphenylacetic acid from 4-hydroxyphenylacetonitrile. A fed batch process designed at 500 ml with five feedings of substrate results in 150 mM product formation using 18 U/ml whole cells nitrilase (16 mg/ml dcw) at 45°C and a volumetric and catalytic productivity of 23 g/l and 4.13 g/g dcw/h -, 758206