2.2.1.6 agriculture inhibitor ZJ0273, i.e. propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino)benzoate at 100 mg/l is the optimal dose of herbicide for rapeseed field 700589 2.2.1.6 agriculture Nicotiana tabacum plants with transplastomic expression of mutants G121A, A122V, or P197S are specifically tolerant to pyrimidinylcarboxylate, imidazolinon, and sulfonylurea/pyrimidinylcarboxylate herbicides, respectively 700807 2.2.1.6 agriculture use of two-point mutated gene of acetolactate synthase from herbicide-resistant rice callus as a selectable marker gene in production of transgenic soybeans. T1 soybeans grown from one regenerated plant after selection of the acetolactate synthase targeting pyrimidinyl-carboxy herbicide bispyribacsodium exhibit herbicide resistance, and the introduction and expression of the gene is confirmed by genetic analysis. The selective culturing is applicable to the production of transgenic soybeans 700729 2.2.1.6 analysis Vis/NIR spectroscopy combined with LS-SVM, i.e. least squares-support vector machine, can be successfully applied for the determination of acetolactate synthase activity and protein content of rapeseed leaves. Vis/NIR spectroscopy is a promising detection technique for the on field applications in oilseed rape 695569 2.2.1.6 biotechnology construction of a vector system for chloroplast transformation with acetolactate synthase, generation of a series of Arabidopsis thaliana mutated acetolactate synthase genes and introduction of constructs with the aminoglycoside 3'-adenyltransferase gene into the Nicotiana tabacum chloroplast genome by particle bombardment 700807 2.2.1.6 biotechnology the reaction specificity of acetolactate synthase from Thermus thermophilus can be redirected to catalyze acetaldehyde formation to develop a thermophilic pyruvate decarboxylase. Quadruple mutant Y35N/K139R/V172A/H474R shows 3.1fold higher acetaldehyde-forming activity than the wild-type mainly because of H474R amino acid substitution, which likely generates two new hydrogen bonds near the thiamine diphosphate-binding site -, 735039 2.2.1.6 brewing unpleasant butterscotch-like flavor in beer due to accumulation of 2,3-butanedione derives from accumulation of mitochondrial matrix-targeted acetohydroxyacid synthase Ilv2 preprotein in the cytosol. Ilv2 preprotein accumulates in the cytosol of petite yeasts. Expression of a mutant allele of the gamma-subunit of the F1-ATPase, ATP3-5, could be an attractive way to reduce diacetyl formation by petite strains 718641 2.2.1.6 drug development acetohydroxyacid synthase (AHAS) of Mycobacterium tuberculosis is a promising target for the development of anti-tuberculosis agents -, 733745 2.2.1.6 drug development AHAS is the target of the sulfonylurea and imidazolinone herbicides 677228 2.2.1.6 drug development the enzyme is a target for development of herbicides, overview 700782