5.2.1.5	biotechnology	the enzyme can be useful for biocatalysis of linoleic acid to conjugated linoleic acid	-, 681731	
5.2.1.5	synthesis	method for converting plant oil into a specific conjugated linoleic acid using a synergistic biocatalytic system based on immobilized Propionibacterium acnes linoleate isomerase and Rhizopus oryzae lipase. Using linoleate isomerase immobilized on D301R anion-exchange resin, under optimal conditions up to 109 g/l trans-10,cis-12-linoleic acid are obtained after incubation of 200 g/l sunflower oil. The conversion ratio of linoleic acid to conjugated linoleic acid is 90.5%	748011	
5.2.1.5	synthesis	production of trans-10,cis-12-linoleic acid as a single isomer by expression of a fusion construct with enhanced green fluorescent protein on the surface of yeast cells. Upon optimization, a maximum yield of trans-10,cis-12-linoleic acid of 4 mg/ml is observed within 20 h using the strain as whole-cell biocatalyst	-, 747336	
5.2.1.5	synthesis	production of trans-10,cis-12-linoleic acid by codon-optimized expression in Mortierella alpina. In presence of 5 microM long-chain acyl-CoA synthetase inhibitor the trans-10,cis-12-linoleic acid content reaches up to 29 mg/l	747384	
5.2.1.5	synthesis	strain Lactobacillus plantarum ZS2058 is able to convert over 50% of linoleic acid to cis-9,trans-11-linoleate and trans9,trans-linoleate. Some intermediates 10-hydroxy-cis-12-octadecenoic acid, 10-oxo-cis-12-octadecenoic acid and 10-oxo-trans-11-octadecenoic acid are found. Expression of the genes coding the multicomponent linoleate isomerase containing myosin-cross-reactive antigen, short-chain dehydrogenase/oxidoreductase and acetoacetate decarboxylase in Escherichia coli leads to the synthesis of cis9,trans-11-linoleate and three kinds of intermediates	-, 748052	
5.2.1.5	synthesis	the enzyme can be useful for biocatalysis of linoleic acid to conjugated linoleic acid	-, 681731