4.6.1.12 analysis high-throughput method for the screening of 2C-methyl-D-erythritol synthase, IspC protein, 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, IspD protein, 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, IspE protein, and 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, IspF protein, against large compound libraries 4.6.1.12 drug development framework for the development of IspF inhibitors to generate lead compounds of therapeutic potential against microbial pathogens 4.6.1.12 medicine the enzyme is a potential target for antimalarial drugs directed at the nonmevalonate pathway of isoprenoid biosynthesis 4.6.1.12 synthesis preparation of 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate in multigram quantities