3.5.1.69	analysis	the enzyme is immobilised on magnetic macroporous cellulose and is used to semisynthesise C17:0 glucosylceramide and C17:0 sulfatide, which are required as internal standards for quantification of the corresponding glycosphingolipids by tandem mass spectrometry	
3.5.1.69	synthesis	a method for generation of novel fluorocarbon derivatives of glycosphingolipids has been developed. SCDase is used to remove the fatty acid from the ceramide moiety, after which a fluorocarbon-rich substituent is incorporated at the free amine of the sphingoid	
3.5.1.69	synthesis	the enzyme is immobilised on magnetic macroporous cellulose and is used to semisynthesise C17:0 glucosylceramide and C17:0 sulfatide, which are required as internal standards for quantification of the corresponding glycosphingolipids by tandem mass spectrometry. A high rate of conversion is achieved for both lipids, 80% for C17:0 sulfatide and 90% for C17:0 glucosylceramide. In contrast to synthesis with a soluble form of the enzyme, use of immobilised SCDase significantly reduces the contamination of the sphingolipid products with other isoforms, so further purification is not necessary	
3.5.1.69	synthesis	the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids	
3.5.1.69	synthesis	sphingolipid ceramide N-deacylase is used for lyso-glycosphingolipid production. Application to large-scale preparation of gamglioside lyso-GM1, overview