1.1.99.35 biofuel production the pyrroloquinoline quinone/glucose dehydrogenase (PQQ-GDH) electrode can be used as bioanode within a biofuel cell. PQQ-GDH is efficiently wired by the polymer polythiophene to the multiwalled carbon nanotubes (MWCNT) electrode 762834 1.1.99.35 biofuel production usage in biofuel cells 762778 1.1.99.35 biotechnology application as biosensor. Application for glucose sensing. s-GDH can be applied for the ultrasensitive detection of PQQ down to picomolar concentrations 762778 1.1.99.35 diagnostics biosensor 762834 1.1.99.35 energy production a (PQQ)-GDH electrode is used as an anode to convert the chemical energy of D-glucose into electrical energy by oxidation of the substrate 724836 1.1.99.35 synthesis expression of enzyme in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli 655363 1.1.99.35 synthesis production of recombinant soluble isoform by expression in Klebsiella pneumoniae at about 18 000 U per l, equal to that achieved in recombinant Escherichia coli. The signal sequence of recombinant PQQGDH-B produced by Klebsiella pneumoniae is correctly processed 702811