6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg - 6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a small amount of cyanophycin is required as a primer 6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium 6.3.2.30 additional information without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis 6.3.2.30 additional information CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview 6.3.2.30 additional information cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers