6.3.1.9	2 glutathione + spermidine + 2 ATP	important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids	
6.3.1.9	2 glutathione + spermidine + 2 ATP	trypanothione synthase overall reaction	
6.3.1.9	2 glutathione + spermidine + 2 ATP	trypanothione synthase overall reaction, trypanothione metabolism overview	
6.3.1.9	2 glutathione + spermidine + 2 ATP	overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase	
6.3.1.9	2 glutathione + spermidine + 2 ATP	overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview	
6.3.1.9	gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP	synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants	
6.3.1.9	gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP	the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione	
6.3.1.9	gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP	-	
6.3.1.9	glutathione + glutathionylspermidine + ATP	-	
6.3.1.9	glutathione + glutathionylspermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei