6.2.1.25 ATP + 2-fluorobenzoate + CoA activation of 2-fluorobenzoate by promiscuous AMP-forming benzoate-CoA ligase 6.2.1.25 ATP + benzoate + CoA - 6.2.1.25 ATP + benzoate + CoA the enzyme catalyzes the initial step of benzoate metabolism 6.2.1.25 ATP + benzoate + CoA enzyme is involved in anaerobic degradation of benzoate 6.2.1.25 ATP + benzoate + CoA activation of benzoate to benzoyl-CoA, a more polar and nondiffusable molecule, decreases the concentration of benzoate in the cell, creating a gradient that favored the uptake of benzoate from the environment 6.2.1.25 ATP + benzoate + CoA first step of anaerobic benzoate degradation 6.2.1.25 additional information benzoate degradation in Azoarcus sp. strain CIB is subject to carbon catabolic repression by some organic acids, indicating the existence of a physiological control that connects the expression of the bzd genes to the metabolic status of the cell