4.6.1.16	additional information	the enzyme is required for tRNA maturation removing an intervening sequence element from the tRNA precursor	
4.6.1.16	additional information	the enzyme is responsible for recognition and excision of nuclear tRNA and all archeal introns	
4.6.1.16	additional information	Aeropyrum pernix expresses precursor-tRNAs with canonical or non-canonical introns at various positions. APE splicing endonuclease removes both types of introns, including the non-canonical introns, without any nucleotide modification. E.g. three tRNAThr species are transcribed and subsequently matured to functional tRNAs. During maturation, introns in two of them are cleaved from standard and non-standard positions, APE-EndA has broad substrate specificity, overview	
4.6.1.16	additional information	AFU-EndA has narrow substrate specificity, overview	
4.6.1.16	pre-tRNA	processing of multiple-intron-containing pretRNA. In these multiple-intron-containing pretRNAs, the introns are nested one inside the other and the pretRNA folds into a conformation that presumably allows the splicing of the last intron only after the splicing of the others. The tRNA splicing endonuclease and the ligase, through a series of cleavages and ligations, should lead to the formation of mature tRNA	
4.6.1.16	pre-tRNA + H2O	-	
4.6.1.16	pretRNA	-	
4.6.1.16	siRNA + H2O	RNA kinase responsible for the phosphorylation at the 5' position of tRNA 3' exons	
4.6.1.16	tRNA + H2O	-	
4.6.1.16	tRNA + H2O	The enzyme is responsible for the excision of introns from nuclear transfer RNA (tRNA) and all archaeal RNAs.