2.8.1.4	L-cysteine + 'activated' tRNA	-	
2.8.1.4	L-cysteine + 'activated' tRNA	sulfur deficient tRNA is the natural substrate	
2.8.1.4	L-cysteine + 'activated' tRNA	enzyme is part of the sulfur-transferase system which forms 4-thiouridylate in tRNA	
2.8.1.4	additional information	Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs	
2.8.1.4	additional information	tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo	
2.8.1.4	sulfide + tRNA-uridine	the enzyme is invoved in biosynthesis of 4-thiouridine in tRNA. 4-Thiouridine, is a modified nucleotide of tRNA that is conserved from bacteria to archaea, where it functions as a sensor for near-UV irradiation