2.1.1.173	additional information	the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY	
2.1.1.173	S-adenosyl-L-methionine + guanine2069 in 23S rRNA	-	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	-	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center	
2.1.1.173	S-adenosyl-L-methionine + guanine2445 in 23S rRNA	Smu472 adds the m2G2445 modification