6.3.1.9	2 glutathione + spermidine + 2 ATP	important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids	
6.3.1.9	2 glutathione + spermidine + 2 ATP	trypanothione synthase overall reaction	
6.3.1.9	2 glutathione + spermidine + 2 ATP	trypanothione synthase overall reaction, trypanothione metabolism overview	
6.3.1.9	2 glutathione + spermidine + 2 ATP	overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase	
6.3.1.9	2 glutathione + spermidine + 2 ATP	overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview	
6.3.1.9	gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP	synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants	
6.3.1.9	gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP	the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione	
6.3.1.9	gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP	-	
6.3.1.9	glutathione + glutathionylspermidine + ATP	-	
6.3.1.9	glutathione + glutathionylspermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei	
6.3.1.9	glutathione + glutathionylspermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9	
6.3.1.9	glutathione + glutathionylspermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei	
6.3.1.9	glutathione + spermidine + 2 ATP	-	
6.3.1.9	glutathione + spermidine + ATP	-	
6.3.1.9	glutathione + spermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei	
6.3.1.9	glutathione + spermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9	
6.3.1.9	glutathione + spermidine + ATP	trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei	
6.3.1.9	glutathione + spermidine + ATP	reaction of EC 6.3.1.8, step one	
6.3.1.9	additional information	phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome	
6.3.1.9	additional information	the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction	
6.3.1.9	additional information	the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8	
6.3.1.9	additional information	the bifunctional trypanothione synthetase-amidase catalyzes biosynthesis and hydrolysis of the glutathione-spermidine adduct trypanothione, the principal intracellular thiolredox metabolite in parasitic trypanosomatids	
6.3.1.9	additional information	in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site	
6.3.1.9	additional information	trypanothione synthetase synthesizes trypanothione from glutathione and spermidine	
6.3.1.9	N1-glutathionylspermidine + glutathione + ATP	step two	
6.3.1.9	N8-glutathionylspermidine + glutathione + ATP	step two	
6.3.1.9	N8-glutathionylspermidine + glutathione + ATP	step two, N8-glutathionylspermidine is the favoured substrate