2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in formation of N-linked oligosaccharides 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in synthesis of peptidoglycan and lipopolysaccharide 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in synthesis of peptidoglycan and teichoic acid 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine key enzyme of encystment 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine enzyme of the Leloir pathway 2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine deficiency mutant fully swollen and some are lysed 2.7.7.23 additional information glycosylation pathway 2.7.7.23 additional information bifunctional enzyme that catalyzes two consecutive reactions leading to the biosynthesis of UDP-N-acetylglucosamine, in the first reaction, GlmU catalyzes the CoA-dependent acetylation of glucosamine-1-phosphate to afford N-acetylglucosamine-1-phosphate, in the second reaction, the UMP moiety from a UTP molecule is transferred to N-acetyl-alpha-D-glucosamine 1-phosphate to give the final product UDP-GlcNAc 2.7.7.23 additional information N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) is a bifunctional enzyme catalyzing the reactions of EC 2.3.1.157, N-acetylglucosamine-1-phosphate uridyltransferase, and 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase, the enzyme catalyzes the two reactions, acetyl transfer and uridyl transfer, at two independent domains, regulation, overview 2.7.7.23 additional information N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) is a pivotal bifunctional enzyme, its N- and C-terminal domains catalyzes uridyltransferase, EC 2.7.7.23, and acetyltransferase, EC 2.3.1.157, activities, respectively 2.7.7.23 additional information the bifunctional role of GlmU arises from two independent domains of the enzyme which possess acetyltransferase, EC 2.3.1.157, and uridyltransferase, EC 2.7.7.23, activities. The acetyltransferase domain, found in the C-terminus of the protein, is responsible for the first step of the reaction, in which an acetyl group is transferred from AcCoA to GlcN-1-P forming GlcNAc-1-P. GlcNAc-1-P then serves as a substrate for the uridyltransferase active site in the N-terminus of the enzyme which forms UDP-GlcNAc 2.7.7.23 additional information the N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase (GlmU) enzyme is a bifunctional enzyme with both acetyltransferase and uridylyltransferase (pyrophosphorylase) activities, catalyzing the reactions of EC 2.3.1.157, N-acetylglucosamine-1-phosphate uridyltransferase, and 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase. Acetyltransfer precedes uridyltransfer with both the acetyltransferase and uridyltransferase reactions taking place in two separable active sites in bifunctional GlmU where the acetyltransferase domain of GlmU does not show homology with its eukaryotic counterpart 2.7.7.23 additional information the N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase (GlmU) enzyme is a bifunctional enzyme with both acetyltransferase and uridylyltransferase (pyrophosphorylase) activities, catalyzing the reactions of EC 2.3.1.157, N-acetylglucosamine-1-phosphate uridyltransferase, and 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase. The synthesis of the two metabolic intermediates N-acetylglucosamine-1-phosphate (GlcNAc-1-P) and UDP-GlcNAc is catalyzed by the C- and N-terminal domains, respectively. UDP-GlcNAc is a key metabolite essential for the synthesis of peptidoglycan, disaccharide linker, arabinogalactan and mycothiols 2.7.7.23 N-acetyl-D-glucosamine 1-phosphate + UTP - 2.7.7.23 N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate - 2.7.7.23 UTP + N-acetyl-alpha-D-galactosamine 1-phosphate - 2.7.7.23 UTP + N-acetyl-alpha-D-galactosamine 1-phosphate reaction of EC 2.7.7.83 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate involved in the synthesis of UDP-N-acetyl-galactoseamine, which is required for cyst wall synthesis 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate - 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the enzyme is involved in biosyntheis of UDP-N-acetylglusosamine 2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms