2.4.1.19	alpha-1,4-glucan + glycosyl acceptor	-	
2.4.1.19	additional information	CGTase can hydrolyze glucan chains, e.g. starch, in a manner similar to alpha-amylases, but differs in its ability to form cyclodextrins as reaction products. Cyclodextrins are formed from starch molecules through intramolecular transglycosylation, i.e. cyclization, and can be made up of 6 to 8 glucan residues, alpha-, beta-, and gamma-cyclodextrin, respectively. The enzyme is multifunctional	
2.4.1.19	additional information	isolation of alkaliphilic Bacillus strains and determination of their phylogenetic and phenotypic characteristics, overview	
2.4.1.19	additional information	CGTase catalyzes the transfer of dextrin units from cyclodextrins or longer dextrins to polyols, such as glycerol, sugars, and flavonoids	
2.4.1.19	additional information	CGTase is an extracellular enzyme capable of converting starch or starch derivatives into cyclodextrins through an intramolecular transglycosylation reaction. Cyclodextrins are cyclic, nonreducing oligoglucopyranose molecules linked via alpha(1,4)-glycosidic bonds	
2.4.1.19	additional information	CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Anaerobranca gottschalkii produces alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus agaradhaerens produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus clarkii produce beta-cyclodextrins, except for strain 290-3 that also produces gamma-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus clarkii produces 80% gamma-cyclodextrins with an overall conversion of starch into cyclodextrins of 14%	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus clarkii produces alpha- and beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus clarkii produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus lichenifoormis produces alpha- and beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus macerans produces alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus megaterium produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus obhensis produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Bacillus strains produce beta-cyclodextrins, strain G-825-6 also produces alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Brevibacillus brevis produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Geobacillus stearothermophilus produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Klebsiella pneumoniae produces alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Paenibacillus campinasensis produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Paenibacillus graminis produces alpha- and beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Paenibacillus illinoisensis produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Paenibacillus pabuli produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Paenibacillus sp. strains produce alpha- and beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from the Bacillus strain G-825-6 produces beta-cyclodextrins and alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from the Bacillus strain produces beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzyme from Thermoanaerobacterium thermosulfurigenes produces alpha- and beta-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzymes from Bacillus circulans strans produce beta-cyclodextrins, except for strain DF 9R that also produces alpha-cyclodextrins	
2.4.1.19	additional information	cyclodextrin glucanotransferases produce a mixture of cyclic alpha-(1,4)-linked oligosaccharides, cyclodextrins, from starch. The enzymes from Thermoanaerobacter sp. strains produce alpha- and beta-cyclodextrins	
2.4.1.19	additional information	the extracelluar enzyme converts starch into non-reducing, cyclic malto-oligosacchrarides called cyclodextrins	
2.4.1.19	potato starch + glycosyl acceptor	worst substrate	
2.4.1.19	soluble starch	-	
2.4.1.19	soluble starch + glycosyl acceptor	-	
2.4.1.19	starch	-	
2.4.1.19	starch + glycosyl acceptor	-	
2.4.1.19	wheat starch + glycosyl acceptor	best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate