1.14.13.128	1,3,7-trimethylxanthine + O2 + NAD(P)H + H+	i.e. caffeine	
1.14.13.128	1,3,7-trimethylxanthine + O2 + NADPH + H+	i.e. caffeine	
1.14.13.128	7-methylxanthine + O2 + NAD(P)H + H+	-	
1.14.13.128	7-methylxanthine + O2 + NAD(P)H + H+	part of the caffeine degradation pathway in Pseudomonas putida	
1.14.13.128	7-methylxanthine + O2 + NADH + H+	-	
1.14.13.128	7-methylxanthine + O2 + NADH + H+	specific substrate	
1.14.13.128	7-methylxanthine + O2 + NADH + H+	the N7-demethylation reaction absolutely requires a unique, tightly bound protein complex composed of NdmC, NdmD, and NdmE. NdmE functions as a noncatalytic subunit that serves a structural role in the complexation of the oxygenase (NdmC) and Rieske domains (NdmD)	
1.14.13.128	7-methylxanthine + O2 + NADH + H+	immediate degradation via the NdmCDE complex	
1.14.13.128	caffeine + O2 + NADH + H+	-	
1.14.13.128	additional information	the metabolite is 1,3-dimethylxanthine, i.e. theophylline, is produced by the demethylation in the 7th position of the purine ring, mainly in fungi, overview	
1.14.13.128	additional information	caffeine, paraxanthine, and theobromine are not demethylated by the enzyme	
1.14.13.128	additional information	no activity toward caffeine or theobromine	
1.14.13.128	additional information	enzyme NdmA catalyzes NADH-dependent N1-demethylation of caffeine to theobromine and theophylline to 3-methylxanthine, and subsequently to 7-methylxanthine, and xanthine. Theobromine is the major product due to highly selective N1-demethylation caffeine by NdmA. But the minor production of paraxanthine from caffeine is due to slight promiscuity of the NdmA towards the N3-position on the caffeine molecule. Direct N-demethylation of caffeine at the N3 position does not occur appreciably with the three N-demethylases involved in caffeine degradation in CBB5