3.1.21.4	DNA + H2O	-	
3.1.21.4	DNA + H2O	primary function is the inactivation of foreign DNA invading bacteria	
3.1.21.4	DNA + H2O	sequence-specific endonucleolytic digestion of infecting DNA	
3.1.21.4	DNA containing F5dC + H2O	perfectly hydrolyzes the DNA containing 5-fluoro-dC	
3.1.21.4	DNA containing F5dU + H2O	perfectly hydrolyzes the DNA containing 5-fluoro-dU	
3.1.21.4	double-stranded DNA + H2O	cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC	
3.1.21.4	double-stranded DNA + H2O	cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC	
3.1.21.4	double-stranded DNA + H2O	-	
3.1.21.4	double-stranded DNA + H2O	the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star	
3.1.21.4	double-stranded DNA + H2O	LmoJ3 recognizes GCNGC	
3.1.21.4	double-stranded DNA + H2O	LmoJ2 recognizes GCWGC (W is A or T) 	
3.1.21.4	double-stranded DNA + H2O	EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively	
3.1.21.4	double-stranded DNA + H2O	cleavage by HindII is blunt, producing fragments with flush ends	
3.1.21.4	dsDNA + H2O	recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands	
3.1.21.4	additional information	the enzyme has the recognition sequence (7/13) GAYN5RTC (14/9). It excises 27 bp, and does not require S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (12/7) RCCGGY (7/12), of which it needs 2 on the substrate to be active. It excises 20 bp, and does not require S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (7/12) GAACN6CTC (13/8), of which it needs 2 on the substrate to be active. It excises 28 bp, and does not require S-adenosyl-L-methionine	
3.1.21.4	additional information	schematic view of the hydrogen-bond interactions of the DNA with each subunit of the protein for the 2TA and 1TA complexes, overview	
3.1.21.4	additional information	BtsI recognizes and digests at GCAGTG(2/0)	
3.1.21.4	additional information	the enzyme has the recognition sequence (10/12) GCAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (7/12) GAACN6TCC (12/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and does not require S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (10/15) ACN4GTAYC (12/7), of which it needs 2 on the substrate to be active. It excises 28 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (10/12) CGAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (8/13) GAGN5CTC (13/8), of which it needs 1 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (9/12) ACN5CTCC (10/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (8/14) CCAN6GT (15/9): It excises 28 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine	
3.1.21.4	additional information	EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI	
3.1.21.4	additional information	no hydrolysis of DNA containing 5-methyl-dC