3.2.1.113 additional information - amino acid composition 135991 3.2.1.113 49000 - 1 * 49000, SDS-PAGE 646583, 646589 3.2.1.113 56000 - x * 56000, SDS-PAGE 646579 3.2.1.113 56920 - theoretical molecular weight. SDS-PAGE, Western blot analysis. A highly purified fraction is obtained after FPLC 677573 3.2.1.113 58000 - x * 58000, SDS-PAGE 715684 3.2.1.113 60000 - His-tagged isozymes MANIa and MANIb, SDS-PAGE 703909 3.2.1.113 63000 - x * 63000, SDS-PAGE 646591 3.2.1.113 64000 - x * 64000, SDS-PAGE 135991 3.2.1.113 65000 - detected in freshly prepared crude microsomal extracts using polyclonal antibodies, during purification the native enzyme, MW 65000, loses a membrane-spanning domain without losing its catalytic activity 646583 3.2.1.113 66000 - gel filtration 753754