4.2.3.12 Ba2+ stimulatory effect, 5 mM greatly accelerates activity 648561 4.2.3.12 Ca2+ 65% of the activity with equal concentration of Mg2+ 648564 4.2.3.12 Ca2+ less than 15% of activity with Mg2+ with 5 mM 648554 4.2.3.12 Ca2+ stimulatory effect and additive effect at Mg2+, 1 mM 648561 4.2.3.12 Co2+ free metal enzyme activity reaches 60% of activity of wild type with 0.50 mM and concentrations higher than 0.15 mM completely inactivates the enzyme. Less than 15% of activity with Mg2+ with 5 mM 648554 4.2.3.12 Mg2+ 75% enhancement 716147 4.2.3.12 Mg2+ free metal enzyme activity is 15% of the wild type with 8 mM, it is not bound to the enzyme 648554 4.2.3.12 Mg2+ necessary for activity 648555, 648559 4.2.3.12 Mg2+ optimum concentration is 8 mM 648564 4.2.3.12 Mg2+ required 648553, 648555