3.5.1.19 Ca2+ activation 246576 3.5.1.19 Co2+ addition of Co2+ after metal removement results in 13% activity 718883 3.5.1.19 Co2+ enhances activity 246588 3.5.1.19 Fe2+ - 679806 3.5.1.19 Fe2+ activates 710862 3.5.1.19 Fe2+ addition of Fe2+ after metal removement results in 13% activity 718883 3.5.1.19 Fe2+ the conserved specific metal ion binding includes one aspartate (D67) and two histidines (H69 and H86) 753679 3.5.1.19 Fe2+ the enzyme contains 0.47 M of Fe2+ per mol of enzyme 718885 3.5.1.19 Fe2+ the enzyme contains a Fe2+ ion in its metal binding site, which is coordinated by an aspartate (D67) and three histidine residues (H69, H75 and H86) 755150 3.5.1.19 Fe2+ the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+ 729184