6.5.1.3 Mg2+ - 1987, 1997 6.5.1.3 Mg2+ activates, optimum: 5-10 mM 662976 6.5.1.3 Mn2+ activates, showing slightly higher activity than Mg2+ 662976 6.5.1.3 additional information calcium, cobalt, copper, cadmium, nickel, and zinc are ineffective 749277 6.5.1.3 Mg2+ dependent on 747437 6.5.1.3 Mg2+ divalent cation required 1985 6.5.1.3 Mn2+ enzyme activity is optimal at 2.5 mM Mn2+ 749277 6.5.1.3 Mg2+ enzyme activity is optimal at 5 mM Mg2+ 749277 6.5.1.3 Mg2+ Km: 0.4 mM, inhibition above 10 mM 1995 6.5.1.3 Mg2+ L1 ligase is an obligate metalloenzyme that is highly specific for Mg2+. It is selected in the presence of 60 mM MgCl2 and functions optimally in Mg2+ concentrations as high as 100 mM 677128