6.5.1.2	Ca2+	10fold lower activation than Mn2+, maximal activity at 2 mM	672811	
6.5.1.2	Ca2+	40% of activity obtained with Mg2+	651212	
6.5.1.2	Ca2+	60% as active as Mg2+ in activation as reported in one study, no activity in another	1909, 1910	
6.5.1.2	Ca2+	activates wild-type enzyme and mutant enzyme DELTA582-667	661789	
6.5.1.2	Ca2+	in presence of Ca2+ MtuLigA is able to carry out the first two steps of ligation reaction that is the transfer of AMP to enzyme from NAD+ and further transfer of it to substrate to form DNA-adenylate intermediate. Ca2+ can not support nick closure activity	677062	
6.5.1.2	Ca2+	supports formation of DNA-adenylate intermediate	653379	
6.5.1.2	Ca2+	the enzyme requires a divalent cation like Ca2+, optimal activity at about 20 mM Ca2+	714854	
6.5.1.2	Co2+	25% of the activity with Mg2+	653388	
6.5.1.2	Co2+	can partially substitute for Mg2+	652175	
6.5.1.2	Co2+	no activity observed in the presence of	728580