4.2.3.9 Co2+ can substitute for Mg2+ over the concentration range of 0.16 -5.0 mM 644244 4.2.3.9 Mg2+ - 644244, 678256, 679218, 680359, 682223, 682226, 694515, 705304 4.2.3.9 Mg2+ divalent cation required, 5 mM Mg2+ preferred 644244 4.2.3.9 Mg2+ enzyme utilizes a trinuclear magnesium cluster to trigger the departure of the diphosphate leaving group, thereby forming an allylic carbocation that typically reacts with one of the remaining sigma-bonds of the substrate 693062 4.2.3.9 Mg2+ required 704204, 747103 4.2.3.9 Mg2+ required for enzyme reaction 691962 4.2.3.9 Mg2+ required, maximal activity at 3 mM 644238 4.2.3.9 Mn2+ - 644244 4.2.3.9 Mn2+ 0.01 mM, can partially replace Mg2+, inhibition above 644238 4.2.3.9 Mn2+ Mn2+ can replace Mg2+ at 0.1-0.2 mM 644244