4.2.1.110 Mg2+ the enzyme contains a structural Mg2+ located in loop region, metal binding site and structure, overview 728150 4.2.1.110 Zn2+ the enzyme contains a structural Zn2+ located in loop regions and two zinc ions at the bottom of two putative active-site clefts in the propeller and the cupin domain, respectively. Catalysis is dependent on these two zinc ions, as their specific removal leads to loss of enzymatic activity, metal binding site and structure, overview 728150