4.1.2.52	Cd2+	0.5 mM chloride salt, 4% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	Co2+	-	702377	
4.1.2.52	Co2+	0.5 mM chloride salt, 100% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	Co2+	required	725493	
4.1.2.52	Cu2+	0.5 mM chloride salt, 1% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	Fe2+	0.5 mM chloride salt, 57% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	Mg2+	0.5 mM chloride salt, 49% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	Mn2+	0.5 mM chloride salt, 99% relative activity, with 4-hydroxy-2-oxopentanoate as substrate	721601	
4.1.2.52	additional information	H45A and H45Q mutant enzymes have 24fold and 69fold higher dissociation constants for Co2+ compared to the wild-type enzyme	722258	
4.1.2.52	additional information	no activity with Ca2+, Fe3+, Cr3+ and Al3+	721601