2.7.7.44 Ca2+ 36% of the activation with Mg2+ 645282 2.7.7.44 Co2+ 40% of the activation with Mg2+ 645282 2.7.7.44 Mg2+ - 670505 2.7.7.44 Mg2+ best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory 645282 2.7.7.44 Mg2+ maximum activity in the presence of Mg2+. 20 mM used in assay conditions 760379 2.7.7.44 Mg2+ required 645283 2.7.7.44 Mn2+ can substitute for Mg2+ in either the forward or the reverse direction, 53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions 645282 2.7.7.44 Zn2+ 37% of the activation with Mg2+ 645282