2.4.1.90 Ca2+ 10 mM used in assay conditions 757639 2.4.1.90 Ca2+ about 50% of the activity with Mn2+ 489555 2.4.1.90 Ca2+ active only with mutant enzymes M334H and M344E, no activity with wild-type enzyme and mutants M344A, M344S, and M344Q 658032 2.4.1.90 Ca2+ can partially replace Mn2+ 489493 2.4.1.90 Ca2+ slight activation 660309 2.4.1.90 Co2+ activates, not as effective as Mn2+ 660309 2.4.1.90 Co2+ activation at 14.9% of the activity with Mn2+ 489526 2.4.1.90 Co2+ less active than Mn2+ with wild-type and mutant enzymes 658032 2.4.1.90 Fe2+ no activation 489555 2.4.1.90 Fe2+ partial activation 489555