1.13.11.6 (NH4)2SO4 the most effective salt, the optimal concentration is 0.035 M 439364 1.13.11.6 Fe2+ solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form 439364 1.13.11.6 HCl during purification of enzyme treatment with acid was used 439364 1.13.11.6 MgCl2 - 439364 1.13.11.6 NaCl - 439364 1.13.11.6 Fe2+ one Fe2+ per active site 439365 1.13.11.6 Fe3+ does not seem to bind to the enzyme 439365 1.13.11.6 Fe2+ requirement 439366, 439370, 439377 1.13.11.6 Fe2+ needed for catalytic activity 649382 1.13.11.6 Fe each monomer contains two iron binding sites. The catalytic iron is buried deep inside the beta-barrel with His51, Glu57, and His95 serving as ligands. The other iron site forms an FeS4 center close to the solvent surface in which the sulfur atoms are provided by Cys125, Cys128, Cys162, and Cys165. The two iron sites are separated by 24 A 672037