1.13.11.6 MgCl2 - 439364 1.13.11.6 NaCl - 439364 1.13.11.6 Fe2+ a non-heme iron-containing enzyme 743276 1.13.11.6 Fe2+ a non-heme iron-containing enzyme, binding structure, overview 741516 1.13.11.6 additional information comparison of the active sites of Fe(III)-h3HAO and Zn(II)-h3HAO enzymes, overview 741516 1.13.11.6 Fe3+ does not seem to bind to the enzyme 439365 1.13.11.6 HCl during purification of enzyme treatment with acid was used 439364 1.13.11.6 Fe each monomer contains two iron binding sites. The catalytic iron is buried deep inside the beta-barrel with His51, Glu57, and His95 serving as ligands. The other iron site forms an FeS4 center close to the solvent surface in which the sulfur atoms are provided by Cys125, Cys128, Cys162, and Cys165. The two iron sites are separated by 24 A 672037 1.13.11.6 Iron enhanced hydrophobicity at the iron center results from the concerted loop movements after the binding of the primary substrate 765043 1.13.11.6 Fe2+ needed for catalytic activity 649382