6.3.1.9 (NH4)2SO4 optimal up to 50 mM 662802 6.3.1.9 KCl optimal up to 50 mM 662802 6.3.1.9 Mg2+ - 661810 6.3.1.9 Mg2+ as MgATP2- 662446, 662802 6.3.1.9 Mg2+ binding sites for N8-glutathionylspermidine , two Mg2+ ions, structure modeling, overview. For the second step of trypanothione synthesis glutathionylspermidine is bound in a way that preferentially allows N1-glutathionylation of N8-glutathionylspermidine, classifying N8-glutathionylspermidine as the favoured substrate 728592 6.3.1.9 Mg2+ MgATP2- required 971 6.3.1.9 Mg2+ required 727114, 727951, 744910, 744964, 746173, 746270 6.3.1.9 Mg2+ required for activity 650657, 652306