6.1.1.7 Co2+ or Mg2+ required 517 6.1.1.7 KCl optimum concentration: 50-100 mM 525 6.1.1.7 Mg2+ - 671126, 675395, 676064, 677045 6.1.1.7 Mg2+ 6-16 mM 525 6.1.1.7 Mg2+ optimum: 5-10 mM 156 6.1.1.7 Mg2+ or Co2+ required 517 6.1.1.7 Mg2+ required 649960, 650000, 714234, 745185 6.1.1.7 Zinc thightly bound 515 6.1.1.7 Zn2+ active site bound by His9, His13, His120, and Cys116, involved in the catalytic reaction 677045 6.1.1.7 Zn2+ potential role of the coordinated zinc in editing substrate specificity 714234 6.1.1.7 Zn2+ removal of Zn2+ leads to over 50% reduced activity, required for conformational stability and flexibility important for the enzymes activity 649422