5.4.2.3	Mg2+	required	
5.4.2.3	Mg2+	divalent metal ion required. Optimal Mg2+ concentration in the presence of dithiothreitol is 10 mM, maximal activity with other metal chelators is obtained with 1 mM Mg2+	
5.4.2.3	Mg2+	1 mM, required	
5.4.2.3	Mg2+	required, Km: 1.2 mM	
5.4.2.3	Mn2+	divalent metal ion required. Mn2+ is less efficient than Mg2+	
5.4.2.3	phosphate	covalently linked to the enzyme. The phosphate-protein linkage is stable in mild acid, at 50 C for up to 2 h. However phosphate is completely released under mild alkaline conditions at 50 C within 1 h